University of Pittsburgh
O'Donnell Lab Yeast Libraries
Saccharomyces cerevisiae Ubiquitin Interaction (ScUbI) Library:
The ScUbI library is a collection of 298 unique ubiquitin-related gene deletion strains arrayed over four, 96-well plates. It was constructed using the Targeted Ubiquitin System (TUS) yeast gene deletion library (Y. Xie, et al (2010) Genes Dev. 24(9):893-903) as a base and expanding it to include additional ubiquitin-related gene deletions. YeastMine, populated by the Saccharomyces Genome Database (SGD) and powered by InterMine, was searched for proteins annotated with ‘ubiquitin’. From the 323 unique gene list returned, duplicates with the TUS library, essential genes and some ubiquitination substrates were removed and the new gene deletion strains were arrayed to generate the ScUbI library. The list of strains in this library can be found in the link below. Please contact Dr. O’Donnell for more information about access to the ScUbI library.
Kinase and Phosphatase Deletion (KinDel) Library:
The KinDel library is comprised of 195 unique strains where each strain has a single, non-essential gene deletion of a protein kinase or protein phosphate. This library began with a kinase and phosphatase list previously created in the Thorner lab (UC Berkeley, J. Patterson 2010 unpublished). YeastMine, populated by the Saccharomyces Genome Database (SGD) and powered by InterMine, was searched for ‘protein kinase’ and ‘protein phosphatase’. From the 208 unique gene list returned, essential genes were removed. SGD annotations of the remaining genes were then checked to ensure they were linked to kinase or phosphatase function. A select group of strains containing gene deletions for six PCL and two PHO deletions, regulators of kinases, was added as a third plate in this library as they are of particular interest to the O’Donnell lab. The list of strains in this library can be found in the link below. Please contact Dr. O’Donnell for more information about access to the KinDel library.
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We gratefully acknowledge the help of Jesse Patterson in generating this library